منابع مشابه
The deficits of matrigel plug assay
| October 2014 | 1018 cells in the matrigel plug (local administration) is not suitable for statically. If the leptin levels were measured before and after matrigel administration, it should have been more meaningful. Because 10-day application of matrigel plug with leptin can’t affect the systemic leptin results. In addition, as shown in recent studies, high fat diet-induced obesity causes inc...
متن کاملA Matrigel-based tube formation assay to assess the vasculogenic activity of tumor cells.
Over the past several decades, a tube formation assay using growth factor-reduced Matrigel has been typically employed to demonstrate the angiogenic activity of vascular endothelial cells in vitro. However, recently growing evidence has shown that this assay is not limited to test vascular behavior for endothelial cells. Instead, it also has been used to test the ability of a number of tumor ce...
متن کاملFrom MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.
متن کاملHigh mobility group box 1 complexed with heparin induced angiogenesis in a matrigel plug assay.
Angiogenesis involves complex processes mediated by several factors and is associated with inflammation and wound healing. High mobility group box 1 (HMGB1) is released from necrotic cells as well as macrophages and plays proinflammatory roles. In the present study, we examined whether HMGB1 would exhibit angiogenic activity in a matrigel plug assay in mice. HMGB1 in combination with heparin st...
متن کاملCells Assemble Invadopodia-Like Structures and Invade into Matrigel in a Matrix Metalloprotease Dependent Manner in the Circular Invasion Assay
The ability of tumor cells to invade is one of the hallmarks of the metastatic phenotype. To elucidate the mechanisms by which tumor cells acquire an invasive phenotype, in vitro assays have been developed that mimic the process of cancer cell invasion through basement membrane or in the stroma. We have extended the characterization of the circular invasion assay and found that it provides a si...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2011
ISSN: 2043-0116
DOI: 10.1038/protex.2011.237